in the "sandwich" elisa technique, what is the function of the capture antibody?

Answers

Answer 1

Answer:

To concentrate the molecule of interest.

Explanation:

Happy Thnskgiving!

Answer 2

The "capture" antibody binds to and retains the analyte from the sample while the rest of the matrix is ​​rinsed away. An enzyme-conjugated "detector" antibody, raised against a different epitope upon analysis, is added to the plate.

What is Elisa Technique?

ELISA stands for enzyme-linked immunosorbent assay. It is a commonly used analytical biochemistry assay. The assay uses a solid-phase type enzyme immunoassay to detect the presence of a ligand in a liquid sample using antibodies directed against the protein to be measured.

It is a plate-based assay technique designed for the detection and quantification of soluble substances such as peptides, proteins, antibodies and hormones. The antigen, called a target macromolecule, is immobilized on a solid surface called a microplate and then complexed with an antibody that is bound to a reporter enzyme.

There are many types of ELISA such as Sandwich ELISA. It measures the antigen between two layers of antibodies called capture and detection antibodies. The target antigen must have at least two antigenic sites capable of binding to the antibody

Thus, the "capture" antibody binds to and retains the analyte from the sample while the rest of the matrix is ​​rinsed away. An enzyme-conjugated "detector" antibody, raised against a different epitope upon analysis, is added to the plate.

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Related Questions

Treatment for fractures includes:
O A. Moving the injured person inside to a bed or couch
B. Leaving the injured part in the position you find it
OC. Applying ice directly to the skin
OD. Washing any wounds

Answers

Answer:

b d

Explanation:

im not 100% sure but thats what  i think it is

Treatment for fractures includes  Leaving the injured part in the position you find it and  Washing any wounds.

What is fracture ?

Fracture is a type of a bone injury resulting it to break or crushed.  It is commonly caused by accidents, disease, great trauma or pressure to the bones.

Fractures can be extremely painful. Normally, fractures required medical attention, different types of fractures Comminuted wherein bones are crushed in pieces, Dislocation where bones or joints are misaligned or dislocated.

Compound fracture is a injury in which the broken bone tears the skin. So in compound fracture as bone tears the skin from inside in cause lots of bleeding and the open wound which makes the site of fracture vulnerable for infections.

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Which type of exercise contributes most to building strong bones?
A.
swimming
B.
lifting weights
C.
walking briskly
D.
jumping rope
SCIENCE

Answers

I think it’s B. Lifting weights

Answer:

C, walking briskly most contributes to building strong bones.

I hope this helped at all.

TIME REMAININ
59:55
In general, a family can be defined as a group of
A individuals who live together and care for each other
B. individuals who share one car or vehicle
C individuals who enjoy each other's company
Please select the best answer from the choices provided.
Ο Α
ОВ
Mark this and return
Save and Exit
Next
Submit

Answers

I think the answers a

Answer:

A

Explanation:

C, could be anyone, your neighbours or even your teachers.

D, is more for friends than family.

a triage team is assessing a client to determine if reported chest pain is a manifestation of angina pectoris or an mi. the nurse knows that a primary distinction of angina pain is?

Answers

Answer:

Relieved by rest and nitroglycerin

Explanation:

Rationale: One characteristic that can differentiate the pain of angina from a myocardial infarction is pain that is relieved by rest and nitroglycerine. There may be some exceptions (unstable angina), but the distinction is helpful especially when combined with other assessment data.

how do you not be a failure in life pls help i am strugleing mentally

Answers

do what makes you happy. sometimes being alone is the best thing you can do so connect with yourself some more, make sure than before anyone else is good that you are too

Answer: get a therapist bc you sound sad

Explanation:

pls get better :)

births that take place prior to ________ weeks of gestation are considered to be preterm births.

Answers

births before 39 weeks.

what is community health? How it can be achieved.
=

Answers

Answer:

The community you live in is part of who you are. Even if you don’t see your neighbors every day, you recognize that the decisions you make impact those around you. You’re all in it together, and you wouldn’t have it any other way!

Improving your community and helping others is often at the top of your mind. So when the phrase “community health” crossed your radar, you had to know more. What is community health? And how does it affect the lives of those in your area?

Community health is the intersection of healthcare, economics and social interaction. Unfortunately, many people are unaware of the role this type of healthcare plays in their everyday lives. Join us as we explore the impact of community health on your neighborhood—and what you can do to improve it.

What is community health?

Community health is a medical specialty that focuses on the physical and mental well-being of the people in a specific geographic region. This important subsection of public health includes initiatives to help community members maintain and improve their health, prevent the spread of infectious diseases and prepare for natural disasters.

“Working at the community level promotes healthy living, helps prevent chronic diseases and brings the greatest health benefits to the greatest number of people in need,” reports the Centers for Disease Control and Prevention (CDC).1

Strong community health requires residents to look beyond themselves and take “collective responsibility,” says Caleb Backe, health and wellness expert at Maple Holistics. “It’s not just about the healthcare system, but focuses on the importance of leading a generally healthy lifestyle in order to protect the community as a whole.”

Community health is inextricably tied to individual wellness. “Good community health equates to healthy people, as a community is the ecosystem or environment in which people live,” says Thomas G. Bognanno, president and CEO of Community Health Charities. “It’s difficult to be healthy personally if your community is unhealthy.”Explanation:

how long do dissolvable stitches last in your mouth

Answers

Answer:

varies based on the type used, but average is 10days.

Explanation:

is sweet tea and coke mixed together good for you or are hey better seperate

Answers

Answer:

what

Explanation:

human should use natural resources wisely and rationally give reason​

Answers

Answer:

¨Natural resources are less consumed and more conserved. The wise and rational uses of natural resources make sustain them for long period of time in nature. It is very essential to balance resources and men exist and we can use them properly in our future.¨

Explanation:

what is the substrate for a antigen and b antigen synthesis?

Answers

Answer:

The blood group A antigen is synthesized by an N-acetylgalactosaminyltransferase that uses a UDP-α-N-acetylgalactosamine (UDP-GalNAc) as sugar donor substrate, while the B antigen is synthesized by a galactosyltransferase that uses a UDP-α-galactose (UDP-Gal) as a sugar donor substrate.

The substrate for A antigen is N-acetylgalactosaminyltransferase which uses a UDP-α-N-acetylgalactosamine (UDP-GalNAc) as sugar donor substrate and the substrate for B Antigen is galactosyltransferase which uses a UDP-α-galactose (UDP-Gal) as a sugar donor substrate.

What are Antigens?

Antigen is any substance which is recognized as foreign particle by the immune system. It is also known as Immunogens. It triggers the Immune system. Antigens can be endogenous, exogenous or autoantigens. Some examples of antigens are pollen, bacteria, viruses, etc.

There are three types of antigens which includes

1. Exogenous antigen - It is foreign to the immune system

2. Endogenous antigen- It is produced by intracellular bacteria and virus which replicates inside the host cell.

3. Autoantigens- It is produced by the host.

Antigen-presenting cells help the immune system triggers its attack. There are three types of antigen-presenting cells in your body. They are

1. Macrophages

2. Dendritic cells

3. B cells

Thus, the substrate for A antigen is N-acetylgalactosaminyltransferase which uses a UDP-α-N-acetylgalactosamine (UDP-GalNAc) as sugar donor substrate and the substrate for B Antigen is galactosyltransferase which uses a UDP-α-galactose (UDP-Gal) as a sugar donor substrate.

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When you try your best to do something good...but you feel like you messed it up? The time you try to help everyone you can because you have such a big heart. But do you EVER get asked "what can I help YOU with?" The time you spend crying just because you feel like nobody is their for you? The time crying because you asked yourself, "Why do people think this is the answer?" When you think of how bad this world is...but there is NOTHING you can do about it. You just think...why do I have to live in a world full of hate... people pulling each other down because that's OK? Girls, starving themselves to be what society things is "good enough". WHEN WILL WE STOP? How many more have to go before we realize we are ALL good enough! But you then think again...am I part of the problem?


Im honestly in a low...but I ALWAYS get through them. I'm not going to do anything to myself AT ALL. (I know some will ask that) This is just a thought of mine. Who can relate? I am always here to talk...so if you need something ASK ME! (and just for the record...im 13)

Answers

Answer:

https://kidshealth.org/en/teens/apologize.html

Explanation:

Answer:

13? You still don't know what the real world is like...but whatever you think, I guess?

Also, I can't really relate, sorry.

What strategy helps prevent diabetes? 20 points

Question 4 options:

maintaining a healthy weight


avoiding exposure to chemicals


drinking lots of water


frequent checkups

Answers

Answer:

A. , maintaining a healthy weight

Explanation:

Answer:

a) Maintaining a healthy weight

Explanation:

Maintaining a healthy weight is the strategy that helps to prevent diabetes.

Losing weight reduces the risk of diabetes. Hence, option (a) is correct answer.

Major strokes are sometimes preceded by:

Answers

Answer:

a bloked artiry or leaking of a blood vessel

Explanation:

i just leared about it today

standard precautions means treating all blood, body fluids, non-intact skin, and mucous membranes as if they could carry bloodborne pathogens and infectious disease.

Answers

The answer is true, in order to avoid infection.

Gina's father has diabetes and takes Insulin. He recently had a liver transplant, after which he was put on anti-rejection medicines. This has made his insulin less effective.
Which term best characterizes Gina's father's situation?
A. synergistic Interaction
B. antagonistic interaction
C. additive interaction​

Answers

Answer:

B. Antagonist interaction.

Explanation:

I hope this helps!

According to the graph, teaching about abstinence declined to about what percentage in 2014?

Answers

From 2000 until 2014, the percentage of schools that required education in human sexuality and abstinence fell to 48% so I would choose 40%

a physically active lifestyle may reduce the risk of:

Answers

heart diseases, many types of cancer, depression, anxiety and other chronic conditions

Chronic disorders such as coronary heart disease, osteoporosis depression, and obesity, amongst others can be reduced.

Why is active lifestyle important?

Physical activities can efficiently reduce the risk of diseases because they increase metabolic activity, thereby boosting the immune system and regeneration of healthy cells in all parts of the body.

Therefore, with this data, we can see that physical activities may reduce the risk of diseases because they are associated with a higher metabolic function and enhanced abilities of the body to face disease conditions such as osteoporosis.

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Muscles make up almost half of a person’s body. What is muscle tissue?

Group of answer choices


tissue that contracts and relaxes to produce movement


tissue that is produced in the spongy part of bones


tissue that is made up mainly of cartilage


tissue that is part of the skeletal system

Answers

Explanation:

tissue that contracts and relaxes to produce movement

is the correct answer.

hope this helps you.

3. What is the mai
n function of the heart?​

Answers

Answer:

Explanation:

renew blood and spread it all to the body

PLZZZ HELPPPPPP
1. A sign of syphilis in the primary stage is:
A. a small painless sore called a chancre.
B. a highly contagious body rash.
C. loss of hair.
D. None of the choices are correct.

2. HIV cannot be spread through:
A. having unprotected sex.
B. breastfeeding.
C. shaking someone's hand.
D. sharing contaminated IV drug needles.

3. Which of the following methods is the one sure way to avoid getting an STD?
A. avoiding sexual intercourse
B. condoms
C. only having oral sex
D. birth control pills

4. Which of the following statements about HIV is false?
A. Symptoms appear immediately after the disease in contracted.
B. It can be many years between infection and the appearance of symptoms.
C. It is spread through the exchange of blood or other bodily fluids.
D. HIV and AIDS have no cure.

5. Which of the following signs needs to be present to diagnose AIDS?
A. Kaposi's sarcoma
B. neurological disorders
C. low white blood cell counts
D. All of these choices are correct.

Answers

Answer: 1.A 2.C 3.B 4.A 5.C

how many calories does the average american consume on thanksgiving?

Answers

The average American will consume about 3,000 calories

a patient is on a mechanical soft diet. which food item choice by the patient will cause the nurse to intervene?

Answers

Given our understanding of this diet type and the reasons behind it, we can confirm that a food item choice by the patient that will cause the nurse to intervene is red meat.

A mechanical soft diet is one in which the patient is allowed to eat solid food, however, the food must be soft and require a minimal amount of chewing. This diet is recommended for patients that have any form of digestive tract illness that may cause difficulty when chewing or swallowing food.

Red meat would cause the nurse to intervene as it presents a great risk for choking or other complications and is prohibited by this diet.

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A patient with diabetes rides a bicycle to and from work every day. which site should the nurse teach the patient to use to administer the morning insulin?

Answers

Answer:

Abdomen

Explanation:

Patients should be taught to not administer insulin into a site that will be exercised because exercise will increase the rate of absorption. The thigh, buttock, and arm are all being worked while riding a bike.

the range of beats per minute you should aim for during exercise is:

Answers

it should be around 220 bpm minus the persons age

Answer:

Around 45- 100 beats.

The range of beats per minute you should aim for during exercise is 45-100 beats.

You don't have to reach exactly that much beats, but it is recommended.It also depends on your age. Say you are 52 years old, the recommended amount of beats is around 85.But, for a average adult, the beats you should aim for is 45-100.

I hope this helped at all.

many different types of mutations can occur within the body

Answers

[tex] \large \text{answer} \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ [/tex]

Mutations / Point Mutations / Frameshift Mutations

Answer:

above answer may help you

Due to the brain’s plasticity, experiences during childhood have a strong impact on who we become. Please select the best answer from the choices provided T F.

Answers

Answer:

This is true!

Answer:

True

Explanation:

Correct on edge 2022

Select the correct answer.

Glenn has a family history of diabetes. Which lifestyle change should he adopt to lower his chances of developing diabetes?

A.

Avoid sugar and sugar products and undergo regular medical tests every month.

B.

Exercise for 30 minutes every day and eat nutritious meals that are low in sugar and saturated fats.

C.

Exercise for 30 minutes every day and undergo regular medical tests every month.

D.

Eat nutritious meals that are low in sugar and saturated fats and take dietary supplements.

Answers

Answer:

B

Exercise for 30 minutes every day and eat nutritious meals that are low in sugar and saturated fats.

what is the difference between tendons and ligaments

Answers

Tendon is link muscle to bones ,But ligament is make two joints dont move thier places.

What organ systems can require mechanical aid to function day to day for patients
with certain kinds of muscular dystrophy?

Answers

respiratory and cardiovascular, This is the rigth answer that should fit for you and I know it would please like.

Answer:

B. respiratory and cardiovascular

Does anyone have the answers to this Midterm im struggling

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