(a) Identify both the cellular component and the location of the component that is responsible for producing the luciferase protein from mRNAs transcribed in the plasmid-containing T lymphocytes. Explain what dictates to the lymphocytes the correct order in which amino acids should be linked to form the luciferase protein.  

(b) Identify the independent variable in the experiment described.  Identify the plasmid that was used as a negative control for luciferase activity.  Justify including the plasmid with the non-CD3γ active promoter in the experiments.


(c) Identify the plasmid that must contain the CD3γ core promoter sequence but the fewest or no negative regulatory sequences. Based on the data in Figure 2, describe the most likely cause of the variation in luciferase activity among the cells that contain plasmids pCD3γ-419, pCD3γ-309, pCD3γ-239, and pCD3γ-199.  Calculate the approximate percent increase in luciferase activity between cells containing plasmid pCD3γ-59 and cells containing plasmid pCD3γ-149. Round to the nearest whole number.


(d) Predict the most likely observed level of luciferase activity if plasmid pCDγ3-789 is introduced into nonlymphoid cells such as a line of kidney tissue cells. Provide reasoning to justify your prediction.

Answers

Answer 1

Ribosomes are the organelles that is resposnible for the production of luciferase.

Where luciferase is produced?

Luciferase is produced by ribosomes in the cytoplasm of the cell or in the cytosol of the lymphocytes. This indicates the sequence of nucleotides in the messager RNA gives message to the amino acid sequence of luciferase.

So we can conclude that ribosomes are the organelles that is resposnible for the production of luciferase.

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Related Questions

The data shows the changes in temperature and the concentration of carbon dioxide (CO2) in Earth’s atmosphere during the past eight hundred thousand years.
Which statement describes the overall relationship between the concentration of carbon dioxide and atmospheric temperature?
A The temperature of Earth increases with a decrease in atmospheric carbon dioxide.
B The temperature of Earth increases with an increase in atmospheric carbon dioxide.
C The temperature of Earth remains unchanged with an increase in atmospheric carbon dioxide.
D The temperature of Earth remains unchanged with a decrease in atmospheric carbon dioxide.

Answers

The answer is c



Because c is always the correct answer

What mechanism is responsible for the targeted integration of a transgene into the host genome?.

Answers

Answer:

illegitimate recombination.

Explanation: Which of the following mechanisms is responsible for the random integration of a transgene into the host genome? Integration of introduced DNA into a random (nonhomologous) occurs by illegitimate recombination.

Non-homologous recombination with the help of a plasmid vector is a mechanism that is responsible for the targeted integration of the transgene into the host genome.

What do you mean by Integration?

Integration may be defined as a process of incorporating the genetic material of a virus or any other transgene into the host genome.

Non-homologous recombination is a type of genetic recombination in which the segment of a gene is transferred to a different set of DNA which is the host genome.

It requires the usage of a plasmid vector that assists in the migration of transgene from the native genome to the host genome.

Therefore, it is well described above.

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At a crime scene you find a piece of glass with a red, dried liquid, and a yellow fiber on the edge lying on the ground. Upon closer inspection, you notice
that there is also a smudged fingerprint crossing the center of the glass. Your job is to plan and carry out an investigation to analyze this evidence. Explain
how you would collect,
preserve and analyze this evidence. What would you hope to find out from this piece of evidence? Indicate whether the glass, red liquid, fingerprint and yellow fiber are each individual or class evidence. Why?

Answers

Answer:

For smaller glass fragments should be placed in a paper packet and then in an envelope. Larger pieces should be wrapped securely in paper or cardboard and then placed in a padded cardboard box to prevent further breakage. For analyzing the glass I would start by comparing the refractive index of the sample to that of the possible source. Examination of samples under a regular microscope or a scanning electron microscope can give detailed information about the sample's physical and chemical characteristics. Since the liquid is already dried all you'd have to do is make sure to package it in paper which works since the glass can go in that. For the fiber I would remove it from the glass and collect it with some trace tape. Then the easiest method to get the fingerprint is called dusting, in which I would use a very fine powder that can stick to the oil in the fingerprint. Once the fingerprint becomes visible, lift it from the surface with clear tape and transfer it to another surface to then take into the laboratory to analyze further.

Glass, the liquid and the fiber would be class evidence while the fingerprint is individual.

Explanation:

The movement of substances from the bloodstream into the tubular nephron is referred to as:.

Answers

Answer:

tubular reabsorption I think

which climate zone has hot summer, cold winter, and average amounts of precipitation throughout the year​

Answers

continental climates

Explain the role of rna polymerase during transcription.

Answers

Answer:

Responsible for copying a DNA sequence into an RNA sequence, during the process of transcription.

How can a protein recognize a specific sequence of dna

Answers

by having a surface that is chemically complimentary to the DNAs

What age is most appropriate for making many repetitive requests?

Two

Ten

Fourteen

Sixteen

Answers

Answer:

I think that the answer is A) Two.

Genes organized into an operon are beneficial to certain bacteria because the operon allows for:.

Answers

Answer:

coordinated regulation of a group of genes that encode proteins with a common function.

Approximately 5% of the cytosine residues are methylated in the genome of any given eukaryote. In what way is dna methylation related to genetic regulation?.

Answers

DNA methylation is related to genetic regulation because this epigenetic mark can turn on/off certain genes.

What is epigenetics?

Epigenetics can be defined as any heritable pattern in gene expression that does not involve different nucleotide sequences.

Epigenetic modifications include DNA methylation and histone modifications (e.g., histone acetylation).

These epigenetic marks are able to turn on or turn off specific genes during differential gene expression.

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What is converted to electricity at geothermal power plants? at geothermal power plants, is converted to electricity.

Answers

Use of steam is the integral part of process in most power plants. In geothermal power plants, thermal energy (heat) from Earth is converted to electricity.

What is geothermal power plants?

Geothermal power plants are used to generate electricity using Earth's thermal energy.

The process requires following steps.

Hot water is pumped under high pressure from deep underground through a well.Heating of water and its movement through a turbine creates thermal energy.In next step, it gets converted to electricity. The steam rotates the turbine that activates a generator which helps in producing electricity.

Thus, it can be concluded as thermal energy is converted to electricity at geothermal power plants.

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Dorsal roots contain axons that bring ______ information to the spinal cord and ventral roots contain axons that bring _______ commands away from the spinal cord.

Answers

Answer:

sensory/motor

Explanation:

Dorsal roots contain axons that bring sensory information to the spinal cord and ventral roots contain axons that bring motor commands away from the spinal cord.

Select the correct text in the passage.
Select the sentence that states an adaptation shown by the animals in the bathypelagic zone.
The bathypelagic zone spans from one to four thousand meters in depth. It is called the midnight zone, as there is total abs
Marine animals in this zone survive on the dead and decaying organic matter sinking from the upper zones. This matter is
detritus. They also prey on other animals. They have large mouths, sharp teeth, and flexible stomachs to accommodate la
Reset
Next

Answers

Answer:

marine animals in this zone survive on the dead and decaying organic matter sinking from the upper zones. this matter is deritus.

Explanation:

Answer:

They have large mouths, sharp teeth, and flexible stomachs to accommodate...

Explanation:

plato

A certain type of butterfly that feeds on the on the green leaves of a plant. Some of the butterflies are green and some are yellow. These butterflies are a favorite food for a certain bird. Which type of butterflies will become more common when these birds move into this ‘environment?

The yellow butterflies
The green butterflies
Both yellow and green butterflies
None

Answers

Answer:

The yellow butterflies will become the "favorite food" for the bird as their color stands out more than the color of green butterflies.

Which of the following statements is true for both solar energy and wind energy? (1 point)
Energy is able to be collected both day and night.
Energy collection equipment can be installed in agricultural areas without affecting crop production.
Power generation using this energy source does not contribute to water pollution.
Power from this energy source can easily be integrated into existing power grid systems.

Answers

Given what we know, we can confirm that Power generation using these energy sources does not contribute to water pollution.

What are solar and wind energy?

Solar and wind energy are clean means to produce electrical energy to power our everyday needs. Solar energy uses specially created panels to harness the heat energy from the sun and produce electricity. Meanwhile, wind energy uses large propellers to harness the wind in order to power a turbine that in change produces electrical energy. By using natural sources to create electricity, these methods do not cause any form of pollution.

Therefore, we can confirm that Power generation using these energy sources does not contribute to water pollution.

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Define cardiac output.

Answers

Answer:

Cardiac output is a term used in cardiac physiology that depicts the volume of blood being pumped by both ventricles of the heart.

A delta is a formation built up by?

Answers

A river delta is a landform created by deposition of sediment that is carried by a river as the flow leaves its mouth and enters slower-moving or stagnant water. This occurs where a river enters an ocean, sea, estuary, lake, reservoir, or (more rarely) another river that cannot carry away the supplied sediment.  

Answer:

Delta is build up by continuous deposition of sediments, which makes the water shallow, causing the landform to rise above the sea level. A delta is divided into four parts, i.e. subaqueous, subaerial, lower delta plain, upper delta plain.

Explanation:

Drag each tile to the correct location. classify the energy sources as renewable or nonrenewable. energy obtained from water springs inside the earth energy obtained from natural gas energy obtained from coal energy obtained from burning plant waste

Answers

Renewable energy sources are the sources which can never be exhausted. This can include natural gas and water from spring in earth. non-renewable are the one which are exhaustible, which include coal energy

What are the types of energy resource?

Renewable sources of energy involves sunlight, water, biomass, etc. This can be replenished naturally.

Nonrenewable sources includes energy from fossils, coal, petroleum, etc. These are the resources if once exhausted can never be replenished or will take millions of years to replenish.

Thus, on this basis one can say that water spring inside the earth, energy from natural gas are renewable sources of energy while energy from coal energy from burning plants is non-renewable energy resource.

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Which sentence from the passage gives the best clue of what organizational pattern is used?a. "they have much larger ears than asian elephants. "b. "elephants have the largest brains of any land mammal. "c. "they are known to be very intelligent animals. "d. "they normally live between 50 and 70 years. "

Answers

'They have much larger ears than asian elephants' is the sentence which describes how organizational pattern is used.

What is Organizational pattern?

This is used to show relationships between supporting details in a passage or text.

The sentence which depicts this from the passage is 'They have much larger ears than asian elephants'.

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Plaque buildup in the arteries of the limbs is called.

Answers

Answer:

Atherosclerosis

A plant can have either brown (B) or red (b) leaves. Brown is the dominant
trait. If a plant has genotype bb, which best describes the plant?

Answers

Answer:

The plant would be red.

Explanation:

It would be red because red leaves are a recessive trait which has lowercase b's so if the genotype for the plant is bb, it would be red. If the plant had BB, it would be brown and if it had Bb it would be brown but, it would be a carrier of the red gene.

How can two organisms with a dominant trait for a specific feature produce an organism with a recessive trait for the same feature?

Answers

Answer:

because one of the organisms is heterozygous.

Explanation:

When an organism is heterozygous it carries both a dominant and a recessive allele therefore two organisms that are dominant for a specific trait can produce an organism with a recessive trait because one of those two organisms is heterozygous.

A car follows a curved highway from point C to point D, covering a distance of
100 kilometers and displacement of 80 kilometers to the south in 2 hours.
What is speed and velocity of the car in kilometers per hour?

Answers

Answer:

102 kilometer

Explanation:

because when something goes kilometers you add the time plus how much kilometers

Progressive muscle relaxation involves deliberate and continuous relaxing of various muscle groups in the body.
a. True
b. False

Answers

Progressive muscle relaxation involves the deliberate and continuous relaxing of various muscle groups in the body.

Why is this statement true?

This statement is true because it accurately describes the technique of progressive muscle relaxation. This is a method for reducing stress in which the individual actively relaxes muscle groups one by one.

Therefore, we can confirm that this statement to in fact be true in that Progressive muscle relaxation involves deliberate and continuous relaxing of various muscle groups in the body.

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Use the drop-down menus to correctly order the steps for setting up a microscope.


Switch to the highest-power objective.


Rotate the lowest-power objective into place.


Use the fine focus knob to sharpen the image.


Be sure the stage is at the lowest setting.


Place the slide on the stage and secure it in place

Answers

Answer:

4

2

5

1

3

Explanation: I just did this right now

Explanation:

there are the answer all the answer are good

What is the measurement of gravity

Answers

Answer:

Gravity is measured by the acceleration that it gives to freely falling objects. At Earth's surface the acceleration of gravity is about 9.8 metres (32 feet) per second per second. Thus, for every second an object is in free fall, its speed increases by about 9.8 metres per second.

Hope it helpz~

describe how glucose formed in a leaf becomes starch stored in a potato tuber

Answers

⚠ANSWER⚠

↪Glucose is usually converted into starch for storage. Starch is better for storage than glucose because it is insoluble. Plants like potatoes and other tubers, and also fruits like the banana store starch for later use. This starch is stored by special organelles, or cell subunits called amyloplasts.

...hope this helps...

Answer:

dehydration synthesis

Explanation:

monomers of the sugar glucose combine to form a single molecule of the sugar maltose. One of the glucose molecules loses an H, the other loses an OH group, and a water molecule is released when the water molecule is lost its no longer soluble hence its stored in the tuber as starch

Halons contain halogens, which are highly reactive with oxygen. please select the best answer from the choices provided t f

Answers

Answer:

IT"S TRUE  

Explanation:

TRUE!

E 2022

Why are none of the outer planets likely to support life similar to that of Earth ?

Answers

Answer:

Because of outer planets' extreme environments, it is improbable that they would contain any life. Outer planets are the farthest from the Sun; as a result, some lack necessary heat to support life, while others are hotter than expected for undetermined reasons.

Explanation:

Please mark me brainlest

Which of the following statements is true regarding physical
changes?
SELECT ANSWER
A: Burning a log of firewood into ashes is an example of a
physical change.
B: physical change results in a change in the
composition of a substance.
C : Physical changes can be reversed.
D Melting sugar in water is an example of a physical
change.

Answers

Answer:

C. Physical change can be reversed.

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Flannery O'connor In this passage, Shiftlet presents himself as ?What makes this passage Ironic? [LINDNER:] They're not rich and fancy people; just hard-working, honest people who don't really have much but those little homes and a dream of the kind of community they want to raise their children in. . . . You've got to admit that a man, right or wrong, has the right to want to have the neighborhood he lives in a certain kind of way. . . . I want you to believe me when I tell you that race prejudice simply doesn't enter into it. It is a matter of the people of Clybourne Park believing, rightly or wrongly, as I say, that for the happiness of all concerned that our Negro families are happier when they live in their own communities.BENEATHA (with a grand and bitter gesture): This, friends, is the Welcoming Committee!A Raisin in the Sun,Lorraine HansberryWhich statement is the best summary of the theme in this passage?A. There is more that unites people than separates them.B. Racism disguised by politeness is still racism.C. The dream of owning a home unites families of all backgrounds.D. Most communities welcome people from different backgrounds. how is the word onomatopoeia pronounced? HELPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPP PLSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS The vertices of PQR are P(6, 1), Q(3,5), and R(11, 11). The length of segment PR is 125 units. Use the coordinates and geometric reasoning to show that PQR is a right triangle.Explain your reasoning and show your work Which movements influenced education during the Enlightenment? Check all that apply. Current 1 of 8.4 A runs for 240 seconds and then stops. Current 2 is 10.5 A. How long does current 2 have to run to deliver the same amount of charge as current 1? 88.2 s 192 s 2016 s 21,000 s What was Cleopatras life like Please ANSWER Amy is wrapping a present that is a rectangular prism. The height is 18 inches, the width is 16 inches and the length is 20 inches. What is the least amount of wrapping paper, in square inches, that Amy needs to wrap the present Why did the united states involve itself in the gulf war?. 3. he took the bad news with surprising and showed no emotion. What was the greatest cause of the French Revolution? Please help!!! Danil, Gabriel and Hadley share some money in the ratios 3:5:9The difference between the amount of money that Gabriel receives and the amount ofmoney that Hadley receives is 196 euros.Work out the amount of money that Danil receives. What is the value of the expression when a = 3 and b = 5?3a+b4471034 I WILL GIVE YOU 20 POINTS TO THOSE WHO ANSWER THIS QUESTION RIGHT NOOOO SCAMS AND EXPLAIN WHY THAT IS THE ANSWER - 2HCI(aq) ZnCl2(aq) + Ha(g) / was given four different hydrochloric acid solutions, each of had a different molarity. He was curious to see what would en if he put a 5 gram piece of zinc metal into 50 ml of each olution. In which HCI solution would the zinc metal have the est rate of reaction? [ think about how you will compare 5.1 5M HCI LOM HCI 1.5M HCIZn(s) + 2HC|(aq) > ZnCl(aq) + H2(g) Bobby was given four different hydrochloric acid solutions, each of which had a different molarity. He was curious to see what would happen if he put a 5 gram piece of zinc metal into 50 ml of each HI solution. In which HI solution would the zinc metal have the quickest rate of reaction? [ think about how you will compare dollars. ] A) .5M HCI B) 1.0M HCI C) 1.5M HCI D) 2.OM HCI What is 56 (-8) = ___ need fast will give brainliest Write the slope-intercept form of equation.9x + 3y = 27 helppppp................................/ PHYSICS GIVING LOTS OF POINTS!!! 100The number of protons in the nucleus of an atom determines the species of the atom, i.e., the element to which the atom belongs. An atom has the same number of protons and neutrons. But the electron number cannot be used instead because electrons are not within the nucleus electrons are negatively charged electrons can be removed from or added to an atom electrons are lighter than protons